Showing 163 of 163 results
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- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.Lab: Nadav Ahituv, UCSFProject: ENCODE
- CRISPRi CRISPR screen in WTC11Homo sapiens WTC11, 14 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Lab: Bing Ren, UCSDProject: ENCODE
- proliferation CRISPR screen in glutamatergic neuronHomo sapiens glutamatergic neuron, 14 days post differentiation genetically modified (CRISPRi) using CRISPR, (insertion) using CRISPR, (insertion) using TALEN inserting M. musculus Neurog2Lab: Yin Shen, UCSFProject: ENCODE
- proliferation CRISPR screen in neural progenitor cellHomo sapiens neural progenitor cell, 3 days post differentiation genetically modified (CRISPRi) using CRISPR, (insertion) using CRISPR, (insertion) using TALEN inserting M. musculus Neurog2Lab: Yin Shen, UCSFProject: ENCODE
- proliferation CRISPR screen in WTC11Homo sapiens WTC11, 0 days post differentiation genetically modified (CRISPRi) using CRISPR, (insertion) using CRISPR, (insertion) using TALEN inserting M. musculus Neurog2Lab: Yin Shen, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 7 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: synthetic elementsLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of ZFAND3 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZFAND3 enhancer.
- MPRA in Neuro-2aElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ultra conserved element UC88.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TCF7L2 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TCF7L2 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT enhancer, using 3' to 5' direction.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of RET locus in Neuro-2aElements cloned into the pGL3 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the RET enhancer.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of MYC locus in HEK293TElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs6983267.
- MPRA of MYC locus in LNCAPElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs11986220.
- MPRA of MSMB locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MSMB promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of IRF6 locus in HaCaTElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF6 enhancer.
- MPRA of IRF4 locus in SK-MEL-28Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF4 enhancer.
- MPRA of HNF4A locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
- MPRA of HBG1 locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBG1 promoter.
- MPRA of HBB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBB promoter.
- MPRA of GP1BB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the GP1BB promoter.
- MPRA of FOXE1 locus in HeLaElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the FOXE1 promoter.
- MPRA of F9 locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the F9 promoter.
- MPRA of BCL11A locus in HELElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the BCL11A enhancer.
- CRISPR cutting CRISPR screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting CRISPR screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting CRISPR screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- CRISPR cutting CRISPR screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 14 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2 originated from WTC11Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in WTC11Homo sapiens WTC11 genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in activated CD4-positive, CD25-positive, alpha-beta regulatory T cellMus musculus strain C57BL/6NJ activated CD4-positive, CD25-positive, alpha-beta regulatory T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 2 cellMus musculus strain C57BL/6NJ activated T-helper 2 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 1 cellMus musculus strain C57BL/6NJ activated T-helper 1 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated naive CD4-positive, alpha-beta T cellMus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- CRISPRi proliferation screen in K562Homo sapiens K562, 14 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Elements selection method: histone modificationsTiling modality: peak tilingLab: Bing Ren, UCSDProject: ENCODE
- CRISPRi proliferation screen in HCT116Homo sapiens HCT116, 14 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Elements selection method: histone modificationsTiling modality: peak tilingLab: Bing Ren, UCSDProject: ENCODE
- CRISPRi proliferation screen in HCT116Homo sapiens HCT116, 14 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Elements selection method: histone modificationsTiling modality: peak tilingLab: Bing Ren, UCSDProject: ENCODE
- CRISPRi proliferation screen in K562Homo sapiens K562, 0 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Elements selection method: histone modificationsTiling modality: peak tilingLab: Bing Ren, UCSDProject: ENCODE
- CRISPRi proliferation screen in HCT116Homo sapiens HCT116, 0 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Elements selection method: histone modificationsTiling modality: peak tilingLab: Bing Ren, UCSDProject: ENCODE
- CRISPRi proliferation screen in HCT116Homo sapiens HCT116, 0 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Elements selection method: histone modificationsTiling modality: peak tilingLab: Bing Ren, UCSDProject: ENCODE
- CRISPR cutting proliferation screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting proliferation screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- CRISPR cutting proliferation screen of MYC locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting FACS screen of MYC locus in K562 with endogenous protein Sort-seq readout of MYCCRISPR screen targeting candidate CREs following FACS high
- CRISPR cutting FACS screen of MYC locus in K562 with endogenous protein Sort-seq readout of MYCCRISPR screen targeting candidate CREs following FACS low
- MPRA in SK-N-SHHomo sapiens SK-N-SH genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in mammary epithelial cellHomo sapiens mammary epithelial cell female genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- perturbation followed by snATAC-seq in GM12878CRISPRi perturbation of GM12878sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in MCF-7CRISPRi perturbation of MCF7sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by scRNA-seq in K562Homo sapiens K562 genetically modified (CRISPRi) using CRISPRLab: Jay Shendure, UWProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM ZK216348 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM ZK216348 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM RU486 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Mapracorat (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM Mapracorat for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM Hydrocortisone for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM GW870086 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM GW870086 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM dexamethasone (CHEBI:41879) for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM CpdA (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM CpdA for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM CORT108297 (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM CORT108297 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM AZD9567 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM AZD9567 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM AZD2906 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM AZD2906 for 4 hours
- perturbation followed by scRNA-seq in K562Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA)Lab: Tim Reddy, DukeProject: ENCODELibrary construction platform: 10X Genomics Chromium Controller
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of a mix of short, medium, and long elements in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of the same set of elements in both the forward and reverse orientations in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements and barcodes cloned into the 3' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements and barcodes cloned into the 3' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 3' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 3' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 5' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 5' UTR of a reporter in a lentiviral vector, using a wild type integrase to allow genomic integration. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. Tested in the same batch as ORI_b2, using primers around the full element and barcode to circumvent the possible detection of artifacts emerging from transcription initiation internal to the upstream element.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as ORI, and serves as an internal control to ORI_full.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the ORI (STARR-seq lacking a minimal promoter) vector, with both elements and barcodes cloned into the 3' UTR. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in the same batch as HSS_b2, using primers around the full element and barcode to circumvent the possible detection of artifacts emerging from transcription initiation internal to the upstream element.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as HSS, and serves as an internal control to HSS_full.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. One of nine methods testing element activity in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- STARR-seq in K562Single human gDNA fragments and concatenated pairs of such fragments were tested for their enhancer activity with eSTARR-seq. The RNAs derived from the transfected plasmids were sequenced and reported here.Elements selection method: transcription start sitesLab: Haiyuan Yu, CornellProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562/Hepg2 enhancers as a pilot library to a larger-scale experiment.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562/Hepg2 enhancers as a pilot library to a larger-scale experiment.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- CRISPR cutting FACS screen of FMR1 locus in glutamatergic neuron with endogenous protein Sort-seq readout of FMR1Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens FMR1, (insertion) using transduction, using CRISPR cutting (pgRNA) for FMR1 locus
- CRISPR cutting FACS screen of APP locus in glutamatergic neuron with endogenous protein Sort-seq readout of APPHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in glutamatergic neuron with endogenous protein Sort-seq readout of SIN3AHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of MECP2 locus in glutamatergic neuron with endogenous protein Sort-seq readout of MECP2Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- CRISPR cutting FACS screen of APP locus in glutamatergic neuron with endogenous protein Sort-seq readout of APPHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in glutamatergic neuron with endogenous protein Sort-seq readout of SIN3AHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of FMR1 locus in WTC11 with endogenous protein Sort-seq readout of FMR1Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens FMR1, (insertion) using transduction, using CRISPR cutting (pgRNA) for FMR1 locus
- CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APPHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3AHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of MECP2 locus in WTC11 with endogenous protein Sort-seq readout of MECP2Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APPHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3AHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of Sox2 locus in F123 with endogenous protein Sort-seq readout of Sox2Mus musculus F123 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for Sox2 locus
- CRISPR cutting FACS screen of Esrrb locus in F123 with endogenous protein Sort-seq readout of EsrrbMus musculus F123 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for Esrrb locus
- CRISPR cutting FACS screen of Sox2 locus in F123 with endogenous protein Sort-seq readout of Sox2Mus musculus F123 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for Sox2 locus
- CRISPR cutting FACS screen of Esrrb locus in F123 with endogenous protein Sort-seq readout of EsrrbMus musculus F123 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for Esrrb locus
- CRISPR cutting FACS screen of Dppa2 locus in F123 with endogenous protein Sort-seq readout of Dppa2Mus musculus F123 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for Dppa2 locus
- CRISPRi proliferation screen of multiple loci in PC-3Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in DU 145Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in NCI-H460Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in A549Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in MDA-MB-231Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in MCF-7Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in HepG2Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in SW620Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in HCT116Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- CRISPRi proliferation screen of multiple loci in K562Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 100 nM dexamethasone (agonist) for 12 hoursLab: Tim Reddy, DukeProject: GGRTreatment: 100 nM dexamethasone (CHEBI:41879) for 12 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 100 nM dexamethasone (agonist) for 8 hoursLab: Tim Reddy, DukeProject: GGRTreatment: 100 nM dexamethasone (CHEBI:41879) for 8 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 100 nM dexamethasone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: GGRTreatment: 100 nM dexamethasone (CHEBI:41879) for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 100 nM dexamethasone (agonist) for 1 hourLab: Tim Reddy, DukeProject: GGRTreatment: 100 nM dexamethasone (CHEBI:41879) for 1 hour
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: GGR
- STARR-seq in MCF-7Homo sapiens MCF-7 genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in HCT116Homo sapiens HCT116 genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in SH-SY5YHomo sapiens SH-SY5Y genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in HepG2Homo sapiens HepG2 genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionLab: Kevin White, UChicagoProject: ENCODE