Functional Characterization Experiment summary for ENCSR319VAS

doi:10.17989/ENCSR319VAS

Summary

Status
released
Assay
STARR-seq
Biosample summary
Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hours
Biosample Type
cell line
Replication type
isogenic
Treatments
1 μM RU486 for 4 hours
Nucleic acid type
RNA
Library starting quantity
75µg
Size range
900-1000
Extraction method
Qiagen RNA extraction
Strand specificity
Strand-specific (reverse)
Controls

Attribution

ENCODE4 project
Lab
Tim Reddy, Duke
Award
UM1HG009428 (Tim Reddy, Duke)
Project
ENCODE
Aliases
tim-reddy:A549-wg-starrseq-RU486
Date submitted
June 11, 2021
Date released
June 17, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
14Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS028BAJENCGM016JFTENCLB285RAE
31Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS556ENLENCGM016JFTENCLB619EZN
24Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS223VDHENCGM016JFTENCLB078BIP
33Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS556ENLENCGM016JFTENCLB766HAW
11Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS028BAJENCGM016JFTENCLB600GZM
44Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS983INBENCGM016JFTENCLB832ZNV
32Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS556ENLENCGM016JFTENCLB493IJU
12Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS028BAJENCGM016JFTENCLB794NWE
13Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS028BAJENCGM016JFTENCLB559RUA
21Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS223VDHENCGM016JFTENCLB118YOB
43Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS983INBENCGM016JFTENCLB730RKA
23Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS223VDHENCGM016JFTENCLB610CQJ
34Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS556ENLENCGM016JFTENCLB649UIN
42Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS983INBENCGM016JFTENCLB256NNX
22Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS223VDHENCGM016JFTENCLB095ECF
41Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursENCBS983INBENCGM016JFTENCLB147XFK