Functional Characterization Experiment summary for ENCSR215YXA

doi:10.17989/ENCSR215YXA

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens HepG2 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of a mix of short, medium, and long elements in parallel.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Controls
Elements references
Elements mapping
ENCSR192JMI

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:shortMedLong-mpra-RNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11HepG2 cell line genetically modified (insertion) using transient transfectionENCBS763SXBENCGM788EQEENCLB536YAW
21HepG2 cell line genetically modified (insertion) using transient transfectionENCBS709WHMENCGM788EQEENCLB950LVT
31HepG2 cell line genetically modified (insertion) using transient transfectionENCBS223TAZENCGM788EQEENCLB292ZLX
41HepG2 cell line genetically modified (insertion) using transient transfectionENCBS949OMHENCGM788EQEENCLB341HPF