Functional Characterization Experiment summary for ENCSR005XEA
doi:10.17989/ENCSR005XEA
Summary
- Status
- released
- Assay
- STARR-seq
- Biosample summary
- Homo sapiens A549 genetically modified (episome) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Treatments
- 1 μM DMSO (CHEBI:28264) for 4 hours
- Nucleic acid type
- RNA
- Library starting quantity
- 75µg
- Size range
- 900-1000
- Extraction method
- Qiagen RNA extraction
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- tim-reddy:A549-wg-starrseq-DMSO
- Date submitted
- June 11, 2021
- Date released
- June 17, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS091FRV | ENCGM016JFT | ENCLB093EMB |
| 2 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS991WDO | ENCGM016JFT | ENCLB544ADF |
| 2 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS991WDO | ENCGM016JFT | ENCLB465LQN |
| 3 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS017SBQ | ENCGM016JFT | ENCLB402TQA |
| 1 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS091FRV | ENCGM016JFT | ENCLB266IVX |
| 4 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS044OXE | ENCGM016JFT | ENCLB492VUT |
| 2 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS991WDO | ENCGM016JFT | ENCLB693AOH |
| 2 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS991WDO | ENCGM016JFT | ENCLB748NOC |
| 1 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS091FRV | ENCGM016JFT | ENCLB985LOU |
| 1 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS091FRV | ENCGM016JFT | ENCLB094XNG |
| 3 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS017SBQ | ENCGM016JFT | ENCLB934LXL |
| 4 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS044OXE | ENCGM016JFT | ENCLB923CLN |
| 3 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS017SBQ | ENCGM016JFT | ENCLB088LMY |
| 3 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS017SBQ | ENCGM016JFT | ENCLB430BRR |
| 4 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS044OXE | ENCGM016JFT | ENCLB376FTS |
| 4 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM DMSO for 4 hours | ENCBS044OXE | ENCGM016JFT | ENCLB828QPK |