Functional Characterization Experiment summary for ENCSR500VLM
doi:10.17989/ENCSR500VLM
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Mus musculus F123 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for Sox2 locus
- Biosample Type
- cell line
- Perturbation type
- CRISPR cutting
- Tiling modality
- full tiling
- Examined loci
- Sox2 (1st percentile)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 710-720
- Platform
- Illumina HiSeq 4000
- Controls
- Elements references
Attribution
ENCODE4 project
- Lab
- Bing Ren, UCSD
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- bing-ren:JL19E_CREST-seq_F123_Sox2_P2
- Date submitted
- January 31, 2020
- Date released
- June 30, 2022
- Functional Characterization Series
- ENCSR749QHJ , ENCSR048THP
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus F123 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) | ENCBS406SAR | ENCGM394IZY, ENCGM568MIS | ENCLB769FJA |
| 4 | 1 | Mus musculus F123 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) | ENCBS314QOV | ENCGM394IZY, ENCGM568MIS | ENCLB591TLK |
| 2 | 1 | Mus musculus F123 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) | ENCBS143LAK | ENCGM394IZY, ENCGM568MIS | ENCLB390ZQL |
| 3 | 1 | Mus musculus F123 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) | ENCBS414ZKJ | ENCGM394IZY, ENCGM568MIS | ENCLB684IWJ |