Functional Characterization Experiment summary for ENCSR202CHZ
doi:10.17989/ENCSR202CHZ
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transduction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned upstream of the reporter's minimal promoter, with barcode located in the 3' UTR of a reporter in a lentiviral vector, using a mutant integrase to prevent genomic integration. One of nine methods testing element activity in parallel.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:53_MT-mpra-RNA
- Date submitted
- December 2, 2020
- Date released
- February 19, 2021
- Functional Characterization Series
- ENCSR033VBW
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS865RQK | ENCGM352MDL | ENCLB646XKW |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS757FXZ | ENCGM352MDL | ENCLB040URD |
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS837XYC | ENCGM352MDL | ENCLB918KZJ |