Functional Characterization Experiment summary for ENCSR689CKC
doi:10.17989/ENCSR689CKC
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction (low MOI), using CRISPR cutting (sgRNA) for MYC locus
- Biosample Type
- cell line
- Perturbation type
- CRISPR cutting
- Tiling modality
- peak tiling
- Examined loci
- MYC (99-100%)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Description
- CRISPR screen targeting candidate CREs following FACS high
- Treatments
- Puromycin (CHEBI:17939)
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
- Elements cloning
- ENCSR903VDF
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:DCC_K562_MYC_GFP_MYC_Screening_FACS_99-100perCent_Experiment
- Date released
- August 30, 2021
- Functional Characterization Series
- ENCSR261PJJ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 10 days after the sample was treated with Puromycin | ENCBS386FXR | ENCGM063WJB, ENCGM885GEZ | ENCLB877WPM |
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 10 days after the sample was treated with Puromycin | ENCBS682AFK | ENCGM063WJB, ENCGM885GEZ | ENCLB647TTO |