1. Functional Characterization Experiments
  2. MPRA
  3. Mus musculus
  4. Neuro-2a

Functional Characterization Experiment summary for ENCSR642DFC

doi:10.17989/ENCSR642DFC

Summary

Status
released
Assay
MPRA
Biosample summary
Mus musculus strain A albino mouse Neuro-2a genetically modified (insertion) using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ultra conserved element UC88.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
unstranded
Controls
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
References
Aliases
nadav-ahituv:UC88-mpra-satMut-RNA
Date released
October 11, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Mus musculus strain A albino mouse Neuro-2a cell line genetically modified (insertion) using transient transfectionENCBS761QWUENCGM402DPZENCLB287RFF
21Mus musculus strain A albino mouse Neuro-2a cell line genetically modified (insertion) using transient transfectionENCBS831HYCENCGM402DPZENCLB454MUN
31Mus musculus strain A albino mouse Neuro-2a cell line genetically modified (insertion) using transient transfectionENCBS247ZIHENCGM402DPZENCLB042NRY

Files

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Output type
Replicates

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