Functional Characterization Experiment summary for ENCSR881ERG

doi:10.17989/ENCSR881ERG

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens HepG2 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of the same set of elements in both the forward and reverse orientations in parallel.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Controls
Elements mapping
ENCSR200NQW

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:FwdRev-mpra-RNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31HepG2 cell line genetically modified (insertion) using transient transfectionENCBS573RKNENCGM788EQEENCLB721OFT
41HepG2 cell line genetically modified (insertion) using transient transfectionENCBS597FXXENCGM788EQEENCLB014BBJ
11HepG2 cell line genetically modified (insertion) using transient transfectionENCBS352PXLENCGM788EQEENCLB654GRT
21HepG2 cell line genetically modified (insertion) using transient transfectionENCBS497PYPENCGM788EQEENCLB116RTI