Functional Characterization Experiment summary for ENCSR259LFO

doi:10.17989/ENCSR259LFO

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens HepG2 genetically modified (insertion) using transient transfection for SORT1 locus
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
unstranded
Controls
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
References
Aliases
nadav-ahituv:SORT1-1-mpra-satMut-RNA
Date released
October 11, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfectionENCBS316VVPENCGM402DPZENCLB877JUQ
21Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfectionENCBS409LQXENCGM402DPZENCLB148XBH
11Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfectionENCBS313OWAENCGM402DPZENCLB698LRS