Functional Characterization Experiment summary for ENCSR463IRX
doi:10.17989/ENCSR463IRX
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562/Hepg2 enhancers as a pilot library to a larger-scale experiment.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:Pilot_9400-mpra-RNA-hepg2
- Date submitted
- February 13, 2020
- Date released
- September 1, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS408HXQ | ENCGM806QIJ | ENCLB541FJC |
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS450NYV | ENCGM806QIJ | ENCLB574VZO |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS280SLR | ENCGM806QIJ | ENCLB953LMJ |