Functional Characterization Experiment summary for ENCSR023WYG

doi:10.17989/ENCSR023WYG

Summary

Status
released
Assay
pooled clone sequencing
Control type
control
Biosample Type
cloning host
Description
Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562/Hepg2 enhancers as a pilot library to a larger-scale experiment.
Nucleic acid type
DNA
Size range
150-250
Fragmentation methods
chemical (Illumina TruSeq)
Elements references

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:Pilot_9400-mpra-pooled-clone_hepg2
Date submitted
February 14, 2020
Date released
September 1, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11ENCLB669KJT