Functional Characterization Experiment summary for ENCSR900AXT
doi:10.17989/ENCSR900AXT
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens WTC11 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Replication type
- isogenic
- Description
- Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- Treatments
- 80 ng/mL tioguanine (CHEBI:9555) for 7 days
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements cloning
- ENCSR617AZY
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- References
- Aliases
- yin-shen:CRISPRi_1X
- Date released
- December 8, 2020
- Functional Characterization Series
- ENCSR534RBC
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens WTC11 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 80 ng/mL tioguanine for 7 days | ENCBS791RZE | ENCGM361RWU, ENCGM171IJG | ENCLB742CVN |
| 1 | 1 | Homo sapiens WTC11 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 80 ng/mL tioguanine for 7 days | ENCBS176KJH | ENCGM361RWU, ENCGM171IJG | ENCLB152TUP |