ENCBS176KJH / cell line

Summary

Status
released
Term name
WTC11
Summary
Homo sapiens WTC11 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 80 ng/mL tioguanine for 7 days
Culture start date
2018-11-05
Culture harvest date
2018-11-29
Passage number
47
Separated from biosample
ENCBS719FSR

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award PI
Yin Shen, UCSF
Submitted by
Xingjie Ren
Project
ENCODE
External resources
Aliases
yin-shen:WTC11_CRISPRi_1_1X6TG

Treatment details

Treatment
80 ng/mL tioguanine (CHEBI:9555) for 7 days
Type
chemical
Purpose
stimulation

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM361RWUCRISPRicharacterizationCRISPR
ENCGM171IJGinsertionexpressiontransduction

Donor information

Status
released
Accession
ENCDO882UJI
Aliases
encode:donor of WTC11
Species
Homo sapiens
Life stage
adult
Age
30 years
Sex
male
Ethnicity
Japanese
External resources