Functional Characterization Experiment summary for ENCSR744ZON
Summary
- Status
- released
- Assay
- STARR-seq
- Biosample summary
- Mus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T cell genetically modified (episome) using transient transfection
- Biosample Type
- primary cell
- Replication type
- isogenic
- Treatments
- 2 μg/mL anti-Interferon-gamma for 3 days, 10 ng/mL Interleukin-2 (UniProtKB:P04351) for 3 days, 2 μg/mL anti-Interleukin-4 for 3 days, 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days [1-1]0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interleukin-4 for 3 days, 2 μg/mL anti-Interferon-gamma for 3 days, 10 ng/mL Interleukin-2 (UniProtKB:P04351) for 3 days [2-1]
- Nucleic acid type
- RNA
- Library starting quantity
- 30µg
- Size range
- 200-400
- Extraction method
- Qiagen RNA extraction
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- tim-reddy:mmTh0-atac-starrseq
- Date submitted
- August 31, 2021
- Date released
- October 5, 2023
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T primary cell genetically modified (episome) using transient transfection treated with 2 μg/mL anti-Interferon-gamma for 3 days, 10 ng/mL Interleukin-2 for 3 days, 2 μg/mL anti-Interleukin-4 for 3 days, 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days | ENCBS851XMW | ENCGM590GEJ | ENCLB170YAK |
| 2 | 1 | Mus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interleukin-4 for 3 days, 2 μg/mL anti-Interferon-gamma for 3 days, 10 ng/mL Interleukin-2 for 3 days | ENCBS258SYH | ENCGM590GEJ | ENCLB709UDK |