Functional Characterization Experiment summary for ENCSR909OSG
doi:10.17989/ENCSR909OSG
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Mus musculus MIN6 genetically modified (insertion) using transient transfection for ZFAND3 locus
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZFAND3 enhancer.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- References
- Aliases
- nadav-ahituv:ZFAND3-mpra-satMut-RNA
- Date released
- October 11, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus MIN6 cell line genetically modified (insertion) using transient transfection | ENCBS148ZFV | ENCGM402DPZ | ENCLB794LPH |
| 2 | 1 | Mus musculus MIN6 cell line genetically modified (insertion) using transient transfection | ENCBS078DWC | ENCGM402DPZ | ENCLB957KRJ |
| 3 | 1 | Mus musculus MIN6 cell line genetically modified (insertion) using transient transfection | ENCBS771OVU | ENCGM402DPZ | ENCLB317DLQ |