Functional Characterization Experiment summary for ENCSR621FIC

doi:10.17989/ENCSR621FIC

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Homo sapiens A549 genetically modified using CRISPR (sgRNA), transduction 14 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 days
Biosample Type
cell line
Perturbation type
interference
CRISPR screen readout
proliferation
Replication type
isogenic
Description
Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
Treatments
2 μg/mL puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL puromycin (CHEBI:17939) for 2 days
Nucleic acid type
RNA
Size range
710-720
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
bing-ren:BC19E_D14_A549
Date submitted
January 31, 2020
Date released
August 6, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21A549 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 14 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 daysENCBS629DTHENCGM554RTS, ENCGM353IMQENCLB546GDK
11A549 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 14 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 daysENCBS549MFHENCGM554RTS, ENCGM353IMQENCLB024HDD