Functional Characterization Experiment summary for ENCSR022GQD
doi:10.17989/ENCSR022GQD
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transduction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:hepg2-mpra-RNA
- Date submitted
- May 20, 2021
- Date released
- September 1, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS227PRD | ENCGM528GJP | ENCLB960BVO |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS397WJZ | ENCGM528GJP | ENCLB642ONP |
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS415QKE | ENCGM528GJP | ENCLB468THH |