Functional Characterization Experiment summary for ENCSR721BJI
doi:10.17989/ENCSR721BJI
Summary
- Status
- released
- Assay
- MPRA (Control MPRA)
- Control type
- control
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transduction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.
- Nucleic acid type
- DNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Platform
- Illumina NextSeq 500
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:hepg2-mpra-DNA
- Date submitted
- May 20, 2021
- Date released
- September 1, 2021
- ENCLB195XPE submitter comment
- This library previously had strand_specificity of "unstranded" but following an upgrade, strand_specificity no longer belongs on DNA libraries and has since been removed.
- ENCLB261YFX submitter comment
- This library previously had strand_specificity of "unstranded" but following an upgrade, strand_specificity no longer belongs on DNA libraries and has since been removed.
- ENCLB993UVP submitter comment
- This library previously had strand_specificity of "unstranded" but following an upgrade, strand_specificity no longer belongs on DNA libraries and has since been removed.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS415QKE | ENCGM528GJP | ENCLB195XPE |
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS227PRD | ENCGM528GJP | ENCLB261YFX |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS397WJZ | ENCGM528GJP | ENCLB993UVP |