Functional Characterization Experiment summary for ENCSR631KBC
doi:10.17989/ENCSR631KBC
Supersedes ENCSR982GCP
Summary
- Status
- released
- Assay
- CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction (low MOI), using CRISPR cutting (sgRNA) for GATA1 locus
- Biosample Type
- cell line
- Perturbation type
- CRISPR cutting
- Tiling modality
- peak tiling
- Replication type
- isogenic
- Description
- CRISPR screen targeting candidate CREs following survival
- Treatments
- Puromycin (CHEBI:17939)
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Elements references
- Elements cloning
- ENCSR579PNC
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:DCC_K562_GATA1_100xCell_Survival_T14_Experiment_2022
- Date released
- October 11, 2022
- Functional Characterization Series
- ENCSR825HCS
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 14 days after the sample was treated with Puromycin | ENCBS735LNU | ENCGM063WJB, ENCGM885GEZ | ENCLB144WMH |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 14 days after the sample was treated with Puromycin | ENCBS368KKY | ENCGM063WJB, ENCGM885GEZ | ENCLB905QBM |