Functional Characterization Experiment summary for ENCSR002UCW

doi:10.17989/ENCSR002UCW

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens HepG2 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in the same batch as HSS_b2, using primers around the full element and barcode to circumvent the possible detection of artifacts emerging from transcription initiation internal to the upstream element.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Controls
Elements references
Elements mapping
ENCSR638VYO

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:HSS_full-mpra-RNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31HepG2 cell line genetically modified (insertion) using transient transfectionENCBS265GZCENCGM822ULAENCLB379BSK
11HepG2 cell line genetically modified (insertion) using transient transfectionENCBS236VIRENCGM822ULAENCLB984DTO
21HepG2 cell line genetically modified (insertion) using transient transfectionENCBS022VSBENCGM822ULAENCLB702DMD