Functional Characterization Experiment summary for ENCSR002UCW
doi:10.17989/ENCSR002UCW
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in the same batch as HSS_b2, using primers around the full element and barcode to circumvent the possible detection of artifacts emerging from transcription initiation internal to the upstream element.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:HSS_full-mpra-RNA
- Date submitted
- December 2, 2020
- Date released
- February 19, 2021
- Functional Characterization Series
- ENCSR033VBW
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS265GZC | ENCGM822ULA | ENCLB379BSK |
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS236VIR | ENCGM822ULA | ENCLB984DTO |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transient transfection | ENCBS022VSB | ENCGM822ULA | ENCLB702DMD |