Functional Characterization Experiment summary for ENCSR325WCA

doi:10.17989/ENCSR325WCA

Summary

Status
released
Assay
proliferation CRISPR screen
Biosample summary
Homo sapiens MCF-7 genetically modified (insertion) using transduction, using CRISPRi (pgRNA) for multiple loci
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
full tiling
Replication type
isogenic
Description
Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
Treatments
2 μg/mL Puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Nucleic acid type
RNA
Size range
710-720
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
bing-ren:BC19E_D14_MCF7
Date submitted
January 31, 2020
Date released
August 6, 2020
Functional Characterization Series
ENCSR147TRG 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 daysENCBS360PYJENCGM554RTS, ENCGM353IMQENCLB763WWL
21Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 daysENCBS763RJUENCGM554RTS, ENCGM353IMQENCLB953QQP