Functional Characterization Experiment summary for ENCSR759LCV
doi:10.17989/ENCSR759LCV
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens glial cell male adult genetically modified (insertion) using transient transfection, expressing RNAi targeting H. sapiens GABPA for TERT locus
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- References
- Aliases
- nadav-ahituv:TERT-GAa-mpra-satMut-RNA
- Date released
- October 11, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens male adult glial primary cell expressing RNAi targeting H. sapiens GABPA, genetically modified (insertion) using transient transfection | ENCBS733SEF | ENCGM118GEM, ENCGM150KFL | ENCLB924FKE |
| 1 | 1 | Homo sapiens male adult glial primary cell expressing RNAi targeting H. sapiens GABPA, genetically modified (insertion) using transient transfection | ENCBS927UKN | ENCGM118GEM, ENCGM150KFL | ENCLB333RVP |
| 2 | 1 | Homo sapiens male adult glial primary cell expressing RNAi targeting H. sapiens GABPA, genetically modified (insertion) using transient transfection | ENCBS819RKG | ENCGM118GEM, ENCGM150KFL | ENCLB603CCF |