1. Functional Characterization Experiments
  2. CRISPR screen
  3. Homo sapiens
  4. WTC11

Functional Characterization Experiment summary for ENCSR071ZGB

Summary

Status
released
Assay
CRISPR screen (CRISPR disruption screen)
Biosample summary
Homo sapiens WTC11 genetically modified using transduction, CRISPR treated with 160 ng/mL tioguanine for 7 days
Biosample Type
cell line
Examined Loci
MSH2, MSH6, MLH1, PMS2, PCNA, HPRT1
Replication type
isogenic
Description
Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
Treatments
160 ng/mL tioguanine (CHEBI:9555) for 7 days
Nucleic acid type
DNA
Controls
Elements references
Elements cloning
ENCSR617AZY

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
yin-shen:CRISPRn_2X
Date released
December 8, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11WTC11 cell line genetically modified (disruption) using CRISPR, genetically modified (insertion) using transduction treated with 160 ng/mL tioguanine for 7 daysENCBS548ITSENCGM464PLI, ENCGM358SHRENCLB698RWF
21WTC11 cell line genetically modified (disruption) using CRISPR, genetically modified (insertion) using transduction treated with 160 ng/mL tioguanine for 7 daysENCBS760MRZENCGM464PLI, ENCGM358SHRENCLB584DGM