Functional Characterization Experiment summary for ENCSR071ZGB
doi:10.17989/ENCSR071ZGB
Summary
- Status
- released
- Assay
- CRISPR screen
- Biosample summary
- Homo sapiens WTC11 genetically modified using CRISPR (sgRNA), transduction treated with 160 ng/mL tioguanine for 7 days
- Biosample Type
- cell line
- Perturbation type
- disruption
- CRISPR screen readout
- proliferation
- Replication type
- isogenic
- Description
- Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- Treatments
- 160 ng/mL tioguanine (CHEBI:9555) for 7 days
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements cloning
- ENCSR617AZY
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:CRISPRn_2X
- Date released
- December 8, 2020
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | WTC11 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using transduction treated with 160 ng/mL tioguanine for 7 days | ENCBS548ITS | ENCGM464PLI, ENCGM358SHR | ENCLB698RWF |
| 2 | 1 | WTC11 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using transduction treated with 160 ng/mL tioguanine for 7 days | ENCBS760MRZ | ENCGM464PLI, ENCGM358SHR | ENCLB584DGM |