Functional Characterization Experiment summary for ENCSR632EPR
doi:10.17989/ENCSR632EPR
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens HepG2 genetically modified (insertion) using transduction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- Strand-specific
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:strands-mpra-RNA
- Date released
- August 17, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS972OVN | ENCGM836MHV | ENCLB750XBA |
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS952IEQ | ENCGM836MHV | ENCLB297KBH |
| 3 | 1 | Homo sapiens HepG2 cell line genetically modified (insertion) using transduction | ENCBS117QYD | ENCGM836MHV | ENCLB825SVI |