Functional Characterization Experiment summary for ENCSR035ODX
doi:10.17989/ENCSR035ODX
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Mus musculus strain NIH3T3 NIH3T3 genetically modified (insertion) using transient transfection for SHH locus
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- References
- Aliases
- nadav-ahituv:ZRS-13h2-mpra-satMut-RNA
- Date released
- October 11, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus strain NIH3T3 NIH3T3 cell line genetically modified (insertion) using transient transfection | ENCBS297MWK | ENCGM575DWZ | ENCLB830JTS |
| 2 | 1 | Mus musculus strain NIH3T3 NIH3T3 cell line genetically modified (insertion) using transient transfection | ENCBS185MKD | ENCGM575DWZ | ENCLB023VEL |
| 3 | 1 | Mus musculus strain NIH3T3 NIH3T3 cell line genetically modified (insertion) using transient transfection | ENCBS971MTR | ENCGM575DWZ | ENCLB317ISS |