Functional Characterization Experiment summary for ENCSR035ODX

doi:10.17989/ENCSR035ODX

Summary

Status
released
Assay
MPRA
Biosample summary
Mus musculus strain NIH3T3 NIH3T3 genetically modified (insertion) using transient transfection for SHH locus
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
unstranded
Controls
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
References
Aliases
nadav-ahituv:ZRS-13h2-mpra-satMut-RNA
Date released
October 11, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Mus musculus strain NIH3T3 NIH3T3 cell line genetically modified (insertion) using transient transfectionENCBS297MWKENCGM575DWZENCLB830JTS
21Mus musculus strain NIH3T3 NIH3T3 cell line genetically modified (insertion) using transient transfectionENCBS185MKDENCGM575DWZENCLB023VEL
31Mus musculus strain NIH3T3 NIH3T3 cell line genetically modified (insertion) using transient transfectionENCBS971MTRENCGM575DWZENCLB317ISS