Functional Characterization Experiment summary for ENCSR113CEG
doi:10.17989/ENCSR113CEG
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- Biosample Type
- in vitro differentiated cells
- Perturbation type
- CRISPR cutting
- Tiling modality
- full tiling
- Examined loci
- SIN3A (1st percentile)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
- Elements cloning
- ENCSR493NRD
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:Low mCherry EX sorted assay_SIN3A
- Date released
- July 19, 2022
- Functional Characterization Series
- ENCSR221WBZ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS778PAZ | ENCGM635WFO, ENCGM232DQE, ENCGM544NZE | ENCLB263UCR |
| 2 | 2 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS903RYW | ENCGM635WFO, ENCGM232DQE, ENCGM544NZE | ENCLB918KVY |