Functional Characterization Experiment summary for ENCSR395WAX

doi:10.17989/ENCSR395WAX

Supersedes ENCSR765TLA

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Homo sapiens K562 genetically modified (insertion) using transduction (low MOI), using CRISPR cutting (sgRNA) for GATA1 locus
Biosample Type
cell line
Perturbation type
CRISPR cutting
Tiling modality
peak tiling
Replication type
isogenic
Description
Control for CRISPR screen targeting candidate CREs
Treatments
Puromycin (CHEBI:17939)
Nucleic acid type
DNA
Elements references
Elements cloning
ENCSR579PNC

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
yin-shen:DCC_K562_GATA1_100xCell_Survival_T0_Experiment_2022
Date released
October 11, 2022
Functional Characterization Series
ENCSR825HCS 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 0 days after the sample was treated with PuromycinENCBS229RRTENCGM063WJB, ENCGM885GEZENCLB639QWX
11Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 0 days after the sample was treated with PuromycinENCBS119DLGENCGM063WJB, ENCGM885GEZENCLB489ONO