Functional Characterization Experiment summary for ENCSR117YGQ

doi:10.17989/ENCSR117YGQ

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Homo sapiens WTC11 genetically modified using CRISPR (sgRNA), transduction treated with 80 ng/mL tioguanine for 7 days
Biosample Type
cell line
Perturbation type
disruption
CRISPR screen readout
proliferation
Replication type
isogenic
Description
Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
Treatments
80 ng/mL tioguanine (CHEBI:9555) for 7 days
Nucleic acid type
DNA
Controls
Elements references
Elements cloning
ENCSR617AZY

Attribution

ENCODE4 project
Lab
Yin Shen, UCSF
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
yin-shen:CRISPRn_1X
Date released
December 8, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11WTC11 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using transduction treated with 80 ng/mL tioguanine for 7 daysENCBS254MAMENCGM464PLI, ENCGM358SHRENCLB219TOG
21WTC11 cell line genetically modified (disruption) using CRISPR (sgRNA), genetically modified (insertion) using transduction treated with 80 ng/mL tioguanine for 7 daysENCBS561NPOENCGM464PLI, ENCGM358SHRENCLB400AKT