ENCBS254MAM / cell line
Summary
- Status
- released
- Term name
- WTC11
- Term ID
- EFO:0009747
- Summary
- Homo sapiens WTC11 cell line genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (sgRNA) treated with 80 ng/mL tioguanine for 7 days
- Culture start date
- 2018-10-24
- Culture harvest date
- 2018-11-19
- Passage number
- 46
- Separated from biosample
- ENCBS993NCH
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award PI
- Yin Shen, UCSF
- Submitted by
- Xingjie Ren
- Project
- ENCODE
- External resources
- Aliases
- yin-shen:WTC11_CRISPRn_1_1X6TG
Treatment details
- Treatment
- 80 ng/mL tioguanine (CHEBI:9555) for 7 days
- Type
- chemical
- Purpose
- stimulation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM464PLI | CRISPR cutting | characterization | CRISPR | ||
ENCGM358SHR | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO882UJI
- Aliases
- encode:donor of WTC11
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 30 years
- Sex
- male
- Ethnicity
- Japanese
- External resources