Functional Characterization Experiment summary for ENCSR049RIU

doi:10.17989/ENCSR049RIU

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens HepG2 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the HSS (STARR-seq) vector, with both elements and barcodes cloned into the 3' UTR. Tested in an independent, second batch as HSS, and serves as an internal control to HSS_full.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Controls
Elements references
Elements mapping
ENCSR638VYO

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:HSS_b2-mpra-RNA
Date submitted
December 2, 2020
Date released
February 19, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11HepG2 cell line genetically modified (insertion) using transient transfectionENCBS143ZWNENCGM822ULAENCLB888RRJ
21HepG2 cell line genetically modified (insertion) using transient transfectionENCBS628ZASENCGM822ULAENCLB638EZJ
31HepG2 cell line genetically modified (insertion) using transient transfectionENCBS930VCWENCGM822ULAENCLB533MIZ