Functional Characterization Experiment summary for ENCSR261RMM

doi:10.17989/ENCSR261RMM

Summary

Status
released
Assay
STARR-seq
Biosample summary
Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours
Biosample Type
cell line
Replication type
isogenic
Treatments
1 μM Hydrocortisone for 4 hours
Nucleic acid type
RNA
Library starting quantity
75µg
Size range
900-1000
Extraction method
Qiagen RNA extraction
Strand specificity
Strand-specific (reverse)
Controls

Attribution

ENCODE4 project
Lab
Tim Reddy, Duke
Award
UM1HG009428 (Tim Reddy, Duke)
Project
ENCODE
Aliases
tim-reddy:A549-wg-starrseq-Hydrocortisone
Date submitted
June 11, 2021
Date released
June 17, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS001CTKENCGM016JFTENCLB338NDL
13Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS021QPJENCGM016JFTENCLB737IQJ
23Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS001CTKENCGM016JFTENCLB692VUD
11Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS021QPJENCGM016JFTENCLB461JZA
32Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS916DKOENCGM016JFTENCLB131LTV
42Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS702UMGENCGM016JFTENCLB766WOU
14Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS021QPJENCGM016JFTENCLB980MQM
22Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS001CTKENCGM016JFTENCLB443NFL
43Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS702UMGENCGM016JFTENCLB064DWB
12Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS021QPJENCGM016JFTENCLB293ZFJ
31Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS916DKOENCGM016JFTENCLB327BQL
24Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS001CTKENCGM016JFTENCLB328YBQ
41Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS702UMGENCGM016JFTENCLB785FKN
33Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS916DKOENCGM016JFTENCLB238COI
34Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS916DKOENCGM016JFTENCLB511AFF
44Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursENCBS702UMGENCGM016JFTENCLB766UWH