Functional Characterization Experiment summary for ENCSR261RMM
doi:10.17989/ENCSR261RMM
Summary
- Status
- released
- Assay
- STARR-seq
- Biosample summary
- Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Treatments
- 1 μM Hydrocortisone for 4 hours
- Nucleic acid type
- RNA
- Library starting quantity
- 75µg
- Size range
- 900-1000
- Extraction method
- Qiagen RNA extraction
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- tim-reddy:A549-wg-starrseq-Hydrocortisone
- Date submitted
- June 11, 2021
- Date released
- June 17, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
2 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS001CTK | ENCGM016JFT | ENCLB338NDL |
1 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS021QPJ | ENCGM016JFT | ENCLB737IQJ |
2 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS001CTK | ENCGM016JFT | ENCLB692VUD |
1 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS021QPJ | ENCGM016JFT | ENCLB461JZA |
3 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS916DKO | ENCGM016JFT | ENCLB131LTV |
4 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS702UMG | ENCGM016JFT | ENCLB766WOU |
1 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS021QPJ | ENCGM016JFT | ENCLB980MQM |
2 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS001CTK | ENCGM016JFT | ENCLB443NFL |
4 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS702UMG | ENCGM016JFT | ENCLB064DWB |
1 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS021QPJ | ENCGM016JFT | ENCLB293ZFJ |
3 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS916DKO | ENCGM016JFT | ENCLB327BQL |
2 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS001CTK | ENCGM016JFT | ENCLB328YBQ |
4 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS702UMG | ENCGM016JFT | ENCLB785FKN |
3 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS916DKO | ENCGM016JFT | ENCLB238COI |
3 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS916DKO | ENCGM016JFT | ENCLB511AFF |
4 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hours | ENCBS702UMG | ENCGM016JFT | ENCLB766UWH |