Functional Characterization Experiment summary for ENCSR694XBP

doi:10.17989/ENCSR694XBP

Summary

Status
released
Assay
proliferation CRISPR screen
Biosample summary
Homo sapiens HepG2 genetically modified (insertion) using transduction, using CRISPRi (pgRNA) for multiple loci
Biosample Type
cell line
Perturbation type
CRISPRi
Tiling modality
full tiling
Replication type
isogenic
Description
Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
Treatments
2 μg/mL Puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Nucleic acid type
RNA
Size range
710-720
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
bing-ren:BC19E_D14_HepG2
Date submitted
January 31, 2020
Date released
August 6, 2020
Functional Characterization Series
ENCSR319WEU 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 daysENCBS451LCJENCGM554RTS, ENCGM353IMQENCLB524CGC
21Homo sapiens HepG2 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 daysENCBS623TRQENCGM554RTS, ENCGM353IMQENCLB685FIM