Functional Characterization Experiment summary for ENCSR119JRG
doi:10.17989/ENCSR119JRG
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- Biosample Type
- in vitro differentiated cells
- Perturbation type
- CRISPR cutting
- Tiling modality
- full tiling
- Examined loci
- MECP2 (1st percentile)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:Low eGFP EX sorted cells assay_MECP2
- Date submitted
- February 1, 2020
- Date released
- July 19, 2022
- Functional Characterization Series
- ENCSR606RFJ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS696HOA | ENCGM120SZX, ENCGM023OJC, ENCGM340BJQ | ENCLB105WQU |
| 2 | 2 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS931EAL | ENCGM120SZX, ENCGM023OJC, ENCGM340BJQ | ENCLB900JCW |