Functional Characterization Experiment summary for ENCSR952RDF
doi:10.17989/ENCSR952RDF
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- Biosample Type
- in vitro differentiated cells
- Perturbation type
- CRISPR cutting
- Tiling modality
- full tiling
- Examined loci
- APP (1st percentile)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
- Elements cloning
- ENCSR783CGW
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:Low mCherry EX sorted assay_APP
- Date submitted
- February 1, 2020
- Date released
- July 19, 2022
- Functional Characterization Series
- ENCSR385YAU
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 2 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS408RJL | ENCGM081YCA, ENCGM880STF, ENCGM501OPY | ENCLB415FCS |
| 1 | 1 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS200IJS | ENCGM081YCA, ENCGM880STF, ENCGM501OPY | ENCLB873SYX |