Functional Characterization Experiment summary for ENCSR364KFC
doi:10.17989/ENCSR364KFC
Summary
- Status
- released
- Assay
- CRISPR screen (Control CRISPR screen)
- Control type
- control
- Biosample summary
- Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- Biosample Type
- in vitro differentiated cells
- Perturbation type
- CRISPR cutting
- Tiling modality
- full tiling
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Elements references
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:Not sorted EX control assay_APP
- Date submitted
- February 1, 2020
- Date released
- July 19, 2022
- Functional Characterization Series
- ENCSR385YAU , ENCSR760PTC
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 2 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS004VDI | ENCGM081YCA, ENCGM880STF, ENCGM501OPY | ENCLB518XKZ |
| 1 | 1 | Homo sapiens male adult (30 years) glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS457ZRQ | ENCGM081YCA, ENCGM880STF, ENCGM501OPY | ENCLB288QIX |