Functional Characterization Experiment summary for ENCSR515OUJ

doi:10.17989/ENCSR515OUJ

Summary

Status
released
Assay
CRISPR screen
Biosample summary
Homo sapiens NCI-H460 genetically modified using CRISPR (sgRNA), transduction 14 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 days
Biosample Type
cell line
Perturbation type
interference
CRISPR screen readout
proliferation
Replication type
isogenic
Description
Growth-based CRISPR screen targeting genomic regions near MYC and MYB genes.
Treatments
2 μg/mL puromycin (CHEBI:17939) for 2 days, 0.2 μg/mL puromycin (CHEBI:17939) for 2 days
Nucleic acid type
RNA
Size range
710-720
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award
UM1HG009402 (Yin Shen, UCSF)
Project
ENCODE
Aliases
bing-ren:BC19E_D14_H460
Date submitted
January 31, 2020
Date released
August 6, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21NCI-H460 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 14 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 daysENCBS175XJFENCGM554RTS, ENCGM353IMQENCLB444KQB
11NCI-H460 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 14 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 daysENCBS933GQAENCGM554RTS, ENCGM353IMQENCLB569QVT