Functional Characterization Experiment summary for ENCSR701WDZ

doi:10.17989/ENCSR701WDZ

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens HEK293T genetically modified (insertion) using transient transfection for HNF4A locus
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
unstranded
Controls
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
References
Aliases
nadav-ahituv:HNF4A-mpra-satMut-RNA
Date released
October 11, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31Homo sapiens HEK293T cell line genetically modified (insertion) using transient transfectionENCBS020JOKENCGM955MLHENCLB874DMI
11Homo sapiens HEK293T cell line genetically modified (insertion) using transient transfectionENCBS108PTIENCGM955MLHENCLB806PAY
21Homo sapiens HEK293T cell line genetically modified (insertion) using transient transfectionENCBS732XWJENCGM955MLHENCLB405KMZ