Functional Characterization Experiment summary for ENCSR459NUS
doi:10.17989/ENCSR459NUS
Summary
- Status
- released
- Assay
- STARR-seq
- Biosample summary
- Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Treatments
- 1 μM dexamethasone (CHEBI:41879) for 4 hours
- Nucleic acid type
- RNA
- Library starting quantity
- 75µg
- Size range
- 900-1000
- Extraction method
- Qiagen RNA extraction
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- tim-reddy:A549-wg-starrseq-Dex
- Date submitted
- June 11, 2021
- Date released
- June 17, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
1 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS862FFO | ENCGM016JFT | ENCLB223WWC |
3 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS483MQT | ENCGM016JFT | ENCLB538NRR |
2 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS906DTK | ENCGM016JFT | ENCLB215IMR |
2 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS906DTK | ENCGM016JFT | ENCLB926FIK |
1 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS862FFO | ENCGM016JFT | ENCLB616RHB |
2 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS906DTK | ENCGM016JFT | ENCLB175GZW |
3 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS483MQT | ENCGM016JFT | ENCLB795XFM |
1 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS862FFO | ENCGM016JFT | ENCLB034UKB |
4 | 2 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS781ZYP | ENCGM016JFT | ENCLB294SGK |
2 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS906DTK | ENCGM016JFT | ENCLB220MOU |
3 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS483MQT | ENCGM016JFT | ENCLB133HJV |
4 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS781ZYP | ENCGM016JFT | ENCLB598VZD |
1 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS862FFO | ENCGM016JFT | ENCLB939GTN |
4 | 4 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS781ZYP | ENCGM016JFT | ENCLB874BHJ |
3 | 3 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS483MQT | ENCGM016JFT | ENCLB699VPG |
4 | 1 | Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hours | ENCBS781ZYP | ENCGM016JFT | ENCLB721XHD |