Functional Characterization Experiment summary for ENCSR138NWM
doi:10.17989/ENCSR138NWM
Summary
- Status
- released
- Assay
- perturbation followed by snATAC-seq
- Biosample summary
- Homo sapiens MCF-7, 6 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (sgRNA)
- Biosample Type
- cell line
- Replication type
- unreplicated
- Description
- CRISPRi perturbation of MCF7s
- Nucleic acid type
- DNA
- Size range
- 100-1000
- Platform
- Illumina NextSeq 550
- Elements references
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- External resources
- Aliases
- will-greenleaf:sp_experiment7
- Date released
- September 17, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 3 | Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA), 6 days post-nucleic acid delivery time | ENCBS634UOB | ENCGM795IKY, ENCGM617AEN | ENCLB981TEC |
| 1 | 2 | Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA), 6 days post-nucleic acid delivery time | ENCBS634UOB | ENCGM795IKY, ENCGM617AEN | ENCLB448CVI |
| 1 | 1 | Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA), 6 days post-nucleic acid delivery time | ENCBS634UOB | ENCGM795IKY, ENCGM617AEN | ENCLB884NYY |
| 1 | 4 | Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA), 6 days post-nucleic acid delivery time | ENCBS634UOB | ENCGM795IKY, ENCGM617AEN | ENCLB498TAE |