ENCBS634UOB / cell line
Summary
- Status
- released
- Term name
- MCF-7
- Term ID
- EFO:0001203
- Summary
- Homo sapiens MCF-7 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA), 6 days post-nucleic acid delivery time
- Description
- CRISPRi perturbation of MCF7s. MCF7 breast cancer cells engineered to express dCas9-KRAB transduced with an 128-sgRNA lentiviral pool, 6 days post-transduction
- Post-nucleic acid delivery time
- 6 days
Attribution
ENCODE4 project
- Lab
- Will Greenleaf, Stanford
- Award PI
- Will Greenleaf, Stanford
- Submitted by
- Tao Wang
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- will-greenleaf:sp_biosample12
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM795IKY | CRISPRi | characterization | CRISPR | ||
| ENCGM617AEN | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO000AAE
- Aliases
- encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 69 years
- Sex
- female
- Health status
- breast cancer (adenocarcinoma)
- Ethnicity
- European
- External resources
- References