Functional Characterization Experiment summary for ENCSR250ZBY
doi:10.17989/ENCSR250ZBY
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- Biosample Type
- cell line
- Perturbation type
- CRISPR cutting
- Tiling modality
- full tiling
- Examined loci
- MECP2 (1st percentile)
- CRISPR screen readout
- endogenous protein Sort-seq
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
- Elements cloning
- ENCSR473BRJ
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:Low eGFP sorted cells assay_MECP2
- Date released
- July 19, 2022
- Functional Characterization Series
- ENCSR855IIN
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 2 | Homo sapiens WTC11 cell line genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS053PSR | ENCGM120SZX, ENCGM023OJC, ENCGM340BJQ | ENCLB228GXC |
| 1 | 1 | Homo sapiens WTC11 cell line genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA) | ENCBS597CSK | ENCGM120SZX, ENCGM023OJC, ENCGM340BJQ | ENCLB449EIJ |