ENCBS053PSR / cell line
Summary
- Status
- released
- Term name
- WTC11
- Term ID
- EFO:0009747
- Summary
- Homo sapiens WTC11 cell line genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, genetically modified (insertion) using transduction, genetically modified using CRISPR cutting (pgRNA)
- Culture start date
- 2018-10-15
- Culture harvest date
- 2018-10-29
- Passage number
- 44
- Separated from biosample
- ENCBS694KTJ
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award PI
- Yin Shen, UCSF
- Submitted by
- Xingjie Ren
- Source
- Li Gan
- Project
- ENCODE
- External resources
- Aliases
- yin-shen:MECP2_2_eGFP
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM120SZX | insertion | tagging | CRISPR |
| |
| ENCGM023OJC | CRISPR cutting | characterization | CRISPR | ||
| ENCGM340BJQ | insertion | expression | transduction |
Donor information
- Status
- released
- Accession
- ENCDO882UJI
- Aliases
- encode:donor of WTC11
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 30 years
- Sex
- male
- Ethnicity
- Japanese
- External resources
Documents
growth protocol
Description excerpt: The SHEN lab Protocol for Culturing Human Pluripotent Stem Cells (hPSCs) in…
- Description
- The SHEN lab Protocol for Culturing Human Pluripotent Stem Cells (hPSCs) in TeSR-E8 Medium. This protocol adapted from the Gan Lab and the Conklin and Karch Lab.
- Submitted by
- Xingjie Ren
- Lab
- Yin Shen, UCSF
- Grant
- UM1HG009402