Functional Characterization Experiment summary for ENCSR300TTB
Summary
- Status
- released
- Assay
- STARR-seq
- Biosample summary
- Mus musculus strain C57BL/6NJ activated T-helper 2 cell genetically modified (episome) using transient transfection
- Biosample Type
- primary cell
- Replication type
- isogenic
- Treatments
- 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interferon-gamma for 4 days, 10 ng/mL Interleukin-2 (UniProtKB:P04351) for 4 days, 2 ng/mL Interleukin-4 (UniProtKB:P07750) for 4 days
- Nucleic acid type
- RNA
- Library starting quantity
- 30µg
- Size range
- 200-400
- Extraction method
- Qiagen RNA extraction
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- tim-reddy:mmTh2-atac-starrseq
- Date submitted
- August 31, 2021
- Date released
- October 5, 2023
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6NJ activated T-helper 2 primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interferon-gamma for 4 days, 10 ng/mL Interleukin-2 for 4 days, 2 ng/mL Interleukin-4 for 4 days | ENCBS074YNO | ENCGM590GEJ | ENCLB183BQU |
| 2 | 1 | Mus musculus strain C57BL/6NJ activated T-helper 2 primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interferon-gamma for 4 days, 10 ng/mL Interleukin-2 for 4 days, 2 ng/mL Interleukin-4 for 4 days | ENCBS768EPQ | ENCGM590GEJ | ENCLB135JWO |