Functional Characterization Experiment summary for ENCSR300TTB

Summary

Status
released
Assay
STARR-seq
Biosample summary
Mus musculus strain C57BL/6NJ activated T-helper 2 cell genetically modified (episome) using transient transfection
Biosample Type
primary cell
Replication type
isogenic
Treatments
0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interferon-gamma for 4 days, 10 ng/mL Interleukin-2 (UniProtKB:P04351) for 4 days, 2 ng/mL Interleukin-4 (UniProtKB:P07750) for 4 days
Nucleic acid type
RNA
Library starting quantity
30µg
Size range
200-400
Extraction method
Qiagen RNA extraction
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Tim Reddy, Duke
Award
UM1HG009428 (Tim Reddy, Duke)
Project
ENCODE
Aliases
tim-reddy:mmTh2-atac-starrseq
Date submitted
August 31, 2021
Date released
October 5, 2023

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Mus musculus strain C57BL/6NJ activated T-helper 2 primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interferon-gamma for 4 days, 10 ng/mL Interleukin-2 for 4 days, 2 ng/mL Interleukin-4 for 4 daysENCBS074YNOENCGM590GEJENCLB183BQU
21Mus musculus strain C57BL/6NJ activated T-helper 2 primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interferon-gamma for 4 days, 10 ng/mL Interleukin-2 for 4 days, 2 ng/mL Interleukin-4 for 4 daysENCBS768EPQENCGM590GEJENCLB135JWO