Functional Characterization Experiment summary for ENCSR585AGE

doi:10.17989/ENCSR585AGE

Summary

Status
released
Assay
STARR-seq
Biosample summary
Homo sapiens K562 genetically modified using transient transfection
Biosample Type
cell line
Replication type
isogenic
Description
Single human gDNA fragments and concatenated pairs of such fragments were tested for their enhancer activity with eSTARR-seq. The RNAs derived from the transfected plasmids were sequenced and reported here.
Nucleic acid type
RNA
Size range
250-650
Fragmentation methods
none
Strand specificity
unstranded
Controls
Elements references

Attribution

ENCODE4 project
Lab
Haiyuan Yu, Cornell
Award
UM1HG009393 (John Lis, Cornell)
Project
ENCODE
Aliases
john-lis:eSTARR2_MYC_RNA
Date submitted
July 8, 2020
Date released
July 13, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31K562 cell line genetically modified (episome) using transient transfectionENCBS575ATBENCGM724VKOENCLB328NEH
21K562 cell line genetically modified (episome) using transient transfectionENCBS623SPPENCGM724VKOENCLB749YTA
11K562 cell line genetically modified (episome) using transient transfectionENCBS601BEQENCGM724VKOENCLB333PBW