Functional Characterization Experiment summary for ENCSR585AGE
doi:10.17989/ENCSR585AGE
Summary
- Status
- released
- Assay
- STARR-seq
- Biosample summary
- Homo sapiens K562 genetically modified (episome) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Single human gDNA fragments and concatenated pairs of such fragments were tested for their enhancer activity with eSTARR-seq. The RNAs derived from the transfected plasmids were sequenced and reported here.
- Nucleic acid type
- RNA
- Size range
- 250-650
- Fragmentation methods
- none
- Strand specificity
- unstranded
- Platform
- Illumina MiSeq
- Controls
- Elements references
- Elements cloning
- ENCSR747QDL
Attribution
ENCODE4 project
- Lab
- Haiyuan Yu, Cornell
- Award
- UM1HG009393 (John Lis, Cornell)
- Project
- ENCODE
- Aliases
- john-lis:eSTARR2_MYC_RNA
- Date submitted
- July 8, 2020
- Date released
- July 13, 2020
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
3 | 1 | Homo sapiens K562 cell line genetically modified (episome) using transient transfection | ENCBS575ATB | ENCGM724VKO | ENCLB328NEH |
2 | 1 | Homo sapiens K562 cell line genetically modified (episome) using transient transfection | ENCBS623SPP | ENCGM724VKO | ENCLB749YTA |
1 | 1 | Homo sapiens K562 cell line genetically modified (episome) using transient transfection | ENCBS601BEQ | ENCGM724VKO | ENCLB333PBW |