Functional Characterization Experiment summary for ENCSR336MKI

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens WTC11 genetically modified (insertion) using transduction
Biosample Type
cell line
Replication type
isogenic
Description
Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
unstranded
Controls
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
Aliases
nadav-ahituv:joint_wtc11-elements-RNA
Date released
December 21, 2023

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens WTC11 cell line genetically modified (insertion) using transductionENCBS541UHIENCGM344VZSENCLB991QNZ
31Homo sapiens WTC11 cell line genetically modified (insertion) using transductionENCBS145GBMENCGM344VZSENCLB699KRT
21Homo sapiens WTC11 cell line genetically modified (insertion) using transductionENCBS640CUXENCGM344VZSENCLB767MAW