1. Functional Characterization Experiments
  2. MPRA
  3. Homo sapiens
  4. glial cell

Functional Characterization Experiment summary for ENCSR782GCT

doi:10.17989/ENCSR782GCT

Summary

Status
released
Assay
MPRA
Biosample summary
Homo sapiens glial cell male adult genetically modified (insertion) using transient transfection for TERT locus
Biosample Type
primary cell
Replication type
isogenic
Description
Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
Nucleic acid type
RNA
Size range
10-20
Fragmentation methods
chemical (Illumina TruSeq)
Strand specificity
unstranded
Controls
Elements references
Elements mappings

Attribution

ENCODE4 project
Lab
Nadav Ahituv, UCSF
Award
UM1HG009408 (Jay Shendure, UW)
Project
ENCODE
References
Aliases
nadav-ahituv:TERT-GBM-mpra-satMut-RNA
Date released
October 11, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
31Homo sapiens male adult glial primary cell genetically modified (insertion) using transient transfectionENCBS186BRYENCGM118GEMENCLB982XHZ
11Homo sapiens male adult glial primary cell genetically modified (insertion) using transient transfectionENCBS663ZQGENCGM118GEMENCLB234GYI
21Homo sapiens male adult glial primary cell genetically modified (insertion) using transient transfectionENCBS617EVFENCGM118GEMENCLB211KIR

Files

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Output type
Replicates

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