Functional Characterization Experiment summary for ENCSR077WEU
doi:10.17989/ENCSR077WEU
Summary
- Status
- released
- Assay
- proliferation CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction (low MOI), using CRISPR cutting (sgRNA) for MYC locus
- Biosample Type
- cell line
- Perturbation type
- CRISPR cutting
- Tiling modality
- peak tiling
- Replication type
- isogenic
- Description
- CRISPR screen targeting candidate CREs following survival
- Treatments
- Puromycin (CHEBI:17939)
- Nucleic acid type
- DNA
- Platform
- Illumina NovaSeq 6000
- Controls
- Elements references
- Elements cloning
- ENCSR903VDF
Attribution
ENCODE4 project
- Lab
- Yin Shen, UCSF
- Award
- UM1HG009402 (Yin Shen, UCSF)
- Project
- ENCODE
- Aliases
- yin-shen:DCC_K562_MYC_GFP_MYC_Screening_survival_Experiment
- Date released
- August 30, 2021
- Functional Characterization Series
- ENCSR042KBK
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 14 days after the sample was treated with Puromycin | ENCBS501PJO | ENCGM063WJB, ENCGM885GEZ | ENCLB871HGX |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction (low MOI), genetically modified using CRISPR cutting (sgRNA) 14 days after the sample was treated with Puromycin | ENCBS892SNM | ENCGM063WJB, ENCGM885GEZ | ENCLB016HLB |