Functional Characterization Experiment summary for ENCSR460LZI
doi:10.17989/ENCSR460LZI
Summary
- Status
- released
- Assay
- MPRA
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transient transfection
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562/Hepg2 enhancers as a pilot library to a larger-scale experiment.
- Nucleic acid type
- RNA
- Size range
- 10-20
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
- Elements references
- Elements mappings
Attribution
ENCODE4 project
- Lab
- Nadav Ahituv, UCSF
- Award
- UM1HG009408 (Jay Shendure, UW)
- Project
- ENCODE
- Aliases
- nadav-ahituv:Pilot_7500-mpra-RNA-k562
- Date submitted
- February 13, 2020
- Date released
- September 1, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transient transfection | ENCBS919ZRZ | ENCGM806QIJ | ENCLB437DSZ |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transient transfection | ENCBS518BIU | ENCGM806QIJ | ENCLB269GBG |
| 3 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transient transfection | ENCBS069NOT | ENCGM806QIJ | ENCLB857UJP |