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- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 7 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: synthetic elementsLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of ZFAND3 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZFAND3 enhancer.
- MPRA in Neuro-2aElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ultra conserved element UC88.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in glial cellElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TERT locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of TCF7L2 locus in MIN6Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TCF7L2 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT enhancer, using 3' to 5' direction.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of RET locus in Neuro-2aElements cloned into the pGL3 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the RET enhancer.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of PKLR locus in K562Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the PKLR promoter.
- MPRA of MYC locus in HEK293TElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs6983267.
- MPRA of MYC locus in LNCAPElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs11986220.
- MPRA of MSMB locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MSMB promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of IRF6 locus in HaCaTElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF6 enhancer.
- MPRA of IRF4 locus in SK-MEL-28Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF4 enhancer.
- MPRA of HNF4A locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
- MPRA of HBG1 locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBG1 promoter.
- MPRA of HBB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HBB promoter.
- MPRA of GP1BB locus in HELElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the GP1BB promoter.
- MPRA of FOXE1 locus in HeLaElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the FOXE1 promoter.
- MPRA of F9 locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the F9 promoter.
- MPRA of BCL11A locus in HELElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the BCL11A enhancer.
- CRISPR cutting CRISPR screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting CRISPR screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting CRISPR screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- CRISPR cutting CRISPR screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYBHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of MYBHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBS1LHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBS1LHomo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBE1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBE1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG2Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG2Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of HBG1Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple lociTiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-RYBP sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-KRAB-WSR7EEE sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-RYBP sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-KRAB-WSR7EEE sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for CRISPRi/dCas9-KRAB-MGA1-MGA2 sparse-tiling proliferation screens at ~7500 cCREs around ~450 essential genes in K562Elements selection method: DNase hypersensitive sitesTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-ZNF705-KRAB/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-RYBP/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-KRAB-WSR7EEE/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen in K562Functional characterization experiment for dCas9-KRAB/CRISPRi tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR dCas proliferation screen in K562Functional characterization experiment for dCas9 tiling proliferation screens at a subset of cCREs around essential genesElements selection method: DNase hypersensitive sitesTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for Cas9/CRISPR fine-mapping proliferation screens at hit and non-hit CTCF motifs in loop anchors (and their partner anchors) from the CTCF growth screenElements selection method: TF binding sitesLoci: CTCFTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for Cas9/CRISPR fine-mapping proliferation screens at hit and non-hit CTCF motifs in loop anchors (and their partner anchors) from the CTCF growth screenElements selection method: TF binding sitesLoci: CTCFTiling modality: peak tilingLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, Cas9/CRISPRk proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR cutting proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, Cas9/CRISPRk proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-KRAB/CRISPRi proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRi proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-KRAB/CRISPRi proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR dCas proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9/CRISPRd proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPR dCas proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9/CRISPRd proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRa proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-VPR/CRISPRa proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- CRISPRa proliferation screen of CTCF locus in K562Functional characterization experiment for tiling, dCas9-VPR/CRISPRa proliferation screens at CTCF motifs in loop anchorsElements selection method: TF binding sitesLoci: CTCFTiling modality: sparse peakLab: Will Greenleaf, StanfordProject: ENCODE
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 14 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2 originated from WTC11Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in WTC11Homo sapiens WTC11 genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in activated CD4-positive, CD25-positive, alpha-beta regulatory T cellMus musculus strain C57BL/6NJ activated CD4-positive, CD25-positive, alpha-beta regulatory T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 17 cellMus musculus strain C57BL/6NJ activated T-helper 17 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 2 cellMus musculus strain C57BL/6NJ activated T-helper 2 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated T-helper 1 cellMus musculus strain C57BL/6NJ activated T-helper 1 cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in activated naive CD4-positive, alpha-beta T cellMus musculus strain C57BL/6NJ activated naive CD4-positive, alpha-beta T cell genetically modified (episome) using transient transfectionElements selection method: accessible genome regionsLab: Tim Reddy, DukeProject: ENCODE
- CRISPR dCas proliferation screen of multiple loci in K562Tiling, dCas9/CRISPRd proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPR dCas proliferation screen of multiple loci in K562Tiling, dCas9/CRISPRd proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRa proliferation screen of multiple loci in K562Tiling, dCas9-VPR/CRISPRa proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRa proliferation screen of multiple loci in K562Tiling, dCas9-VPR/CRISPRa proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPR cutting proliferation screen of multiple loci in K562Tiling, Cas9/CRISPRk proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPR cutting proliferation screen of multiple loci in K562Tiling, Cas9/CRISPRk proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRi proliferation screen of multiple loci in K562Tiling, dCas9-KRAB/CRISPRi proliferation screen at the GATA1, MYB, and ZMYND8 loci
- CRISPRi proliferation screen of multiple loci in K562Tiling, dCas9-KRAB/CRISPRi proliferation screen at the GATA1, MYB, and ZMYND8 loci
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modificationsLab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in pancreas organoidHomo sapiens organoid with pancreatic ductal adenocarcinoma; pancreas genetically modified (episome) using transient transfectionElements selection method: accessible genome regions, histone modifications, DNase hypersensitive sitesLab: Kevin White, UChicagoProject: ENCODE
- CRISPR cutting proliferation screen of GATA1 locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting proliferation screen of GATA1 locus in K562Control for CRISPR screen targeting candidate CREs
- CRISPR cutting proliferation screen of MYC locus in K562CRISPR screen targeting candidate CREs following survival
- CRISPR cutting FACS screen of MYC locus in K562 with endogenous protein Sort-seq readout of MYCCRISPR screen targeting candidate CREs following FACS high
- CRISPR cutting FACS screen of MYC locus in K562 with endogenous protein Sort-seq readout of MYCCRISPR screen targeting candidate CREs following FACS low
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA of multiple loci in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection for multiple loci
- MPRA in HCT116Homo sapiens HCT116, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HCT116Homo sapiens HCT116, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in A549Homo sapiens A549, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in mammary epithelial cellHomo sapiens mammary epithelial cell female genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in K562Homo sapiens K562 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in GM12878Homo sapiens GM12878 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- perturbation followed by snATAC-seq in GM12878CRISPRi perturbation of GM12878sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in MCF-7CRISPRi perturbation of MCF7sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by snATAC-seq in K562CRISPRi perturbation of K562sLab: Will Greenleaf, StanfordProject: ENCODE
- perturbation followed by scRNA-seq in K562Homo sapiens K562 genetically modified (CRISPRi) using CRISPRLab: Jay Shendure, UWProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in K562Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test K562 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM ZK216348 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM ZK216348 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM RU486 (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM RU486 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Mapracorat (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM Mapracorat for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM Hydrocortisone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM Hydrocortisone for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM GW870086 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM GW870086 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM dexamethasone (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM dexamethasone (CHEBI:41879) for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM CpdA (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM CpdA for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM CORT108297 (antagonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM CORT108297 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM AZD9567 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM AZD9567 for 4 hours
- STARR-seq in A549Homo sapiens A549 genetically modified (episome) using transient transfection treated with 1 μM AZD2906 (agonist) for 4 hoursLab: Tim Reddy, DukeProject: ENCODETreatment: 1 μM AZD2906 for 4 hours
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in Jurkat with PrimeFlow readout of PPIFHomo sapiens Jurkat genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in BJAB with PrimeFlow readout of PPIFHomo sapiens BJAB genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in GM12878 with PrimeFlow readout of PPIFHomo sapiens GM12878 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi Flow-FISH screen of PPIF locus in THP-1 with PrimeFlow readout of PPIFHomo sapiens THP-1 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for PPIF locusElements selection method: DNase hypersensitive sitesLoci: PPIFTiling modality: peak tilingLab: Jesse Engreitz, StanfordProject: ENCODE
- CRISPRi proliferation screen in OCI-AML2CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in OCI-AML2 acute myeloid leukemia cellsTiling modality: sparse peakLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi proliferation screen in K562CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen a subset of putative non-coding regulatory elements identified in human K562 leukemia cells from whole genome discovery screen (validation). This validation screen was performed in K562 leukemia cellsTiling modality: sparse peakLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi proliferation screen in K562CRISPR-dCas9-based epigenomic regulatory element screening (CERES) technology to screen all >100,000 putative non-coding regulatory elements defined by open chromatin sites in human K562 leukemia cells for their role in regulating essential cellular processes (whole genome).Tiling modality: peak tilingLab: Tim Reddy, DukeProject: ENCODE
- perturbation followed by scRNA-seq in K562Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA)Lab: Tim Reddy, DukeProject: ENCODELibrary construction platform: 10X Genomics Chromium Controller
- STARR-seq in K562Homo sapiens K562 genetically modified (episome) using transient transfectionLab: Tim Reddy, DukeProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CAPRIN1CAPRIN1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE
- CRISPRi Flow-FISH screen in K562 with HCR-FlowFISH readout of CAPRIN1CAPRIN1Tiling modality: full tilingLab: Pardis Sabeti, BroadProject: ENCODE